碩士 / 國立臺灣海洋大學 / 食品科學系 / 98 / Oxidative stress is now recognized as a common pathology that affects up to half of all infertile men. This study extracted powder of Ascophyllum nodosum by ethanol, acetone, supercritical fluid extract (SFE) and ethanol SFE respectively, and obtained ANE, ANA, ANS and ANC. ANA contained the most fucoxanthin content. And ANE contained the highest level of total polyphenolic content, also demonstrated the strong DPPH radical scavenging activity and reducing power. The ANE, ANA, ANS and ANC did not exert any cytotoxic effect on RAW264.7 under the tested concentration (200 ug/mL). Four kind of extracts remarkablely decreased superoxide anion and intracellular ROS content of macrophage induced by LPS. To evaluate the protective effects of ANE against Cisplatin (CP) -induced reproductive toxicity in hamsters. ANE reduced the extent of CP-induced sperm concentration, abnormality and enhanced sperm motility and mitochondrial membrane potential. Treatment with ANE restored the control level of malondialdehyde (MDA) and intracellular reactive oxygen species (ROS) in the CP-treated testis. The CP injection also induced decline in the levels of superoxide dismutase (SOD) were significantly reversed to control levels in groups where CP was preceded by the administration of ANE. Our results showed the histopathological findings of testes in short term treatment. This study clearly indicates that CP-treatment impaired markedly testicular function, and ANE treatment increased the activities of testicular antioxidant enzymes and restored sperm characteristics. The possible potential beneficial effects of ANE were particularly focused on fucoxanthin, total polyphenol and flavonoid compounds. In our studies, ANE have been shown to exhibit strong antioxidant activity which is attributed to quenching and scavenging free radicals. The protective effects of tested brown algae extract are, therefore, suggested to be mediated by their potent antioxidant activities, and provided significant amelioration of oxidative stress parameters.
| Identifer | oai:union.ndltd.org:TW/098NTOU5253013 |
| Date | January 2010 |
| Creators | Ming-Chou Hsieh, 謝明洲 |
| Contributors | Zwe-Ling Kong, 龔瑞林 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 121 |
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