碩士 / 國立臺灣海洋大學 / 生物科技研究所 / 98 / Gas6 and protein S are the bridge proteins between apoptotic cells and phagocytes. The receptors of Gas6 and protein S on the surface of apoptotic cells belong to the TAM family (Tyro-3/Axl/Mer) tyrosine kinases. The goal of our study is to investigate the role of bridge protein Gas6 and protein S in early zebrafish development . In our study, we have detected the expression of gas6, protein S, axl and tyro-3 gene in the zebrafish brain by in situ hybridization during early zebrafish development. To further analyze the functions of Gas6 and protein S, we have anaylzed the effects of overexpressing Gas6 and protein S in glial cells in zebrafish embryos. Our data showed that a significant number of neuronal cell death could be observed at 24 hpf in zebrafish embryos. In addition, co-overexpression of Axl-GFP and Tyro-3-GFP either with Gas6 or protein S also had resulted in similar but more even severe neuronal cell death; most of the GFP-positive neuronal cells disappeared at 48 hpf. Based on our data, we propose that overexpression of Gas6 and protein S would cause abnormalities in the development of nervous system of zebrafish embryos. In our model, the secretion of Gas6 or protein S by glial cells would lead to the attraction of surrounding apoptotic cells to the normal neurons that possessed Axl receptor, through interactions with Gas6 and protein S. As a result, these apoptotic cells which are phagocytosed by microglia would also affect the neuronal cells in an indirect fashion.
| Identifer | oai:union.ndltd.org:TW/098NTOU5613013 |
| Date | January 2010 |
| Creators | Tzu-Lin Lin, 林子琳 |
| Contributors | Chang-Jen Huang, 黃銓珍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
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