碩士 / 國立臺灣大學 / 微生物與生化學研究所 / 98 / Candida albicans is an opportunistic pathogen in human normal flora. Candida albicans are able to produce both superficial and systemic infections, in immunocompromised hosts. The overall incidence of candidemia has increased persistently worldwide during the second half of the 20th century. In recent years, fungal infections has become one of the leading cause of nosocomial infection. Moreover, drug-resistance is a growing problem largely due to the widespread use of antifungal agents in medicine. Therefore, it is necessary to develop alternative antimicrobial methods which are effective in the treatment of multi-drug resistant fungal infections.
In this study, we found chitosan can augment the photodynamic inactivation (PDI) mediated by TBO and Ce6 against Candida albicans with a 30-min incubation after PDI. At conditions where the PDI could kill the microbe for about 2 to 4-log scale, the addition of chitosan at as low as 0.25% for 30 minutes after the PDI could further eradicate the Candida albicans (originally was 107 CFU/ml). However, without PDI treatment, chitosan alone did not exert significant antimicrobial activity for the for 30-minute incubation. Similar results were also found in drug-resistant strains and the biofilm of Candida albicans. These results indicate that the synergistic effect of chitosan worked after the fungal damage induced by PDI.
| Identifer | oai:union.ndltd.org:TW/098NTU05381063 |
| Date | January 2010 |
| Creators | Po-Han Chang, 張博涵 |
| Contributors | 陳進庭 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 62 |
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