碩士 / 臺北醫學大學 / 保健營養學研究所 / 98 / Sepsis is a common clinical disease induced by infection and multiple organ dysfunction syndrome (MODS) is the main cause of death in the late phase of septic shock. Overproduction of pro-inflammatory mediators and mitochondrial dysfunction are thought to play important roles in pathogenesis of MODS. This study investigated the effects of dietary fish oil on inflammatory response and hepatic mitochondrial function in a cecal ligation and puncture (CLP)-induced septic mice model. Male ICR mice were assigned to a control group (C) and the fish oil group (FO) (n=60). Mice in the C group were fed with a semi-purified diet with 10% soybean oil, and those in the FO group was fed with a fish oil diet (2.5% fish oil+ 7.5% soybean oil; w/w). The ratio of n-3/n-6 fatty acid was 1:2 in FO group. Three weeks later, blood, peritoneal lavage fluid (PLF), livers, lung, kidney, and intestine were harvested at 0, 6 and 24 h after CLP, respectively. Compared with C group, the FO group showed a lower prostaglandin (PG) E2 and tumor necrosis factor (TNF)-α in PLF. Also, plasma levels of TNF-α and nitrite were lower at 6 and 24 h after CLP. The FO group had lower organs myeloperoxidase (MPO) activities at 6 h after CLP. The transcriptional activity of peroxisome proliferators-activated receptor (PPAR)-γ was up-regulated while NF-κB p65 subunit, iNOS protein expression and the concentration of nitrotyrosine were significantly decreased in FO group than C group in liver homogenate after CLP. Hepatic mitochondrial respiratory chain enzyme activities were suppressed during early stage of sepsis in C group. The rotenone-sensitive NADH cytochrome c reductase, succinate cytochrome c reductase and the cytochrome c oxidase activities were higher in the FO group at 6h after CLP. The morphology showed enlargement of hepatic mitochondria during early stage of sepsis. These results suggest that mice fed with fish oil up-regulate the transcriptional activity of PPAR-γ, reduce pro-inflammatory mediator levels and maintain hepatic mitochondrial electron-transport chain enzyme activities in septic mice.
| Identifer | oai:union.ndltd.org:TW/098TMC05217009 |
| Date | January 2010 |
| Creators | Cheng-Chung Li, 李正中 |
| Contributors | 葉松鈴 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 49 |
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