博士 / 國立陽明大學 / 生化暨分子生物研究所 / 98 / Zebrafish is a popular model for genetic and developmental studies, yet very little is known about the mechanism of zebrafish sex determination due to the lack of discernible sex chromosomes and the difficulty of distinguishing the sex of juvenile fish. To alleviate these problems, we generated fish with predictable sex trait by methyltestosterone treatment followed by natural mating. Histology and gene expressions analysis in these juvenile fish with known gender was characterized. Our genetic selection scheme matches the prediction that female-dominant genetic factors are required to determine zebrafish sex. We also found the first sign of zebrafish sex differentiation to be ovarian gonocyte proliferation and differentiation at 10-12 days post-fertilization (dpf). Sex dimorphic expression of cyp19a1a and sox9a, the female and male somatic cell markers, respectively, was also observed at three weeks of age before the appearance of testicular morphology. Besides, we found cyp19a1b was expressed in radial glial cells just peripheral to the brain ventricles in ventral telencephalon, preoptic area, and hypothalamus. Estrogens could upregulate the expression of cyp19a1b in larval brain. We have generated transgenic zebrafish lines expressing GFP driven by cyp19a1b promoter, which exhibited fluorescence signals in adult radial glial cells and in estrogen induced fish larvae. This line provides a tool to further characterize radial glial progenitor cells as well as evaluation of xenoestrogen effects.
| Identifer | oai:union.ndltd.org:TW/098YM005107047 |
| Date | January 2010 |
| Creators | Sok-Keng Tong, 湯淑敬 |
| Contributors | Bon-chu Chung, 鍾邦柱 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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