碩士 / 元培科技大學 / 生物技術研究所 / 98 / High incidence rates of cancer in recent years, patient that caused discomfort on side effects and damage of normal cells for radiotherapy. Many researches indicated that the natural antioxidants provided free radicals scavenging ability, antioxidant activity and anti-cancer effect. The radio-protective and radio-sensitivity of catechin, EGCG, gallic acid and 4 samples of purple bud tea ( PTE ), black tea ( TTES No. 8, BTE ), oolong tea ( TTES No.12, MTE ), Spirulina fermentated products (SFP) on the two cell lines (IEC-6 intestinal normal cells and CT-26 colon cancer cells) by Co-60 irradiation were investigated in this study The results showed that 125 μM of 3 catechins (catechin, EGCG and gallic acid) exhibited cytotoxic on IEC-6 cells, as same as 200 μg / mL of four extracts. The cytotoxicity in the order were gallic acid> EGCG> catechin. Gallic acid and PTE showed the highest toxicity. The cytotoxicity on the CT-26 cells were EGCG> gallic acid> catechin, and four samples were PTE> BTE> MTE> SFP.
The clonogenic cell survival assay found that the catechin or MTE treated IEC-6 cell with after Co-60 irradiation（6 Gy）, the survival fraction were 67.94 ± 13.28 and 32.17 ± 25.00 %, respectively. Catechin of radio-protective effect was best; other gallic acid and EGCG were caused large number of apoptosis and necrosis. On the treated of EGCG, Gallic acid, PTE, BTE, MTE or SFP of CT-26 colorectal cells with Co-60 irradiation（2 Gy）, its survival fraction were 0% that indicated CT-26 cells have radiation-sensitive effect. Thus, according to this result that In vitro cell experiments showed the best curative effect of PTE on colorectal cancer with treatment. The composition of PTE had abundant anthocyanins, EGCG and gallic acid induced radiation-sensitive. The present study suggest that the treatment of colorectal cancer on the future, use the principle of synergistic effects of radio-sensitive materials with radiotherapy, reduce the patient radiotherapy dose and taking catechin and SPF further protect normal cells.
| Identifer | oai:union.ndltd.org:TW/098YUST5108005 |
| Creators | Tse-Zung Liao, 廖澤蓉 |
| Contributors | Chih-Cheng Lin, Chia-Chieh Chen, 林志城, 陳家杰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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