碩士 / 元智大學 / 化學工程與材料科學學系 / 98 / Bio-energy and biodiesel has been approved to reduce the greenhouse effect and secure the energy supply. However, the waste glycerol derived from biodiesel production became an environmental issue. An investigation of mixotrophic cultivation of Chlorella sp. by addition of carbon supply in respect of cell growth and lipid accumulation was carried out. The results indicated 35％ of crude lipid produced from either glucose, glycerol or crude glycerol supplement. The biomass was found low in a range between 0.2~0.4 g L-1 DCW. Hence, Chlorella sp. was concluded as low efficiency of carbon utilization under mixotrophic cultivation. In order to improve lipid production and greenhouse gas reduction, the response surface methodology（RSM）was employed to study the optimal condition of lipid accumulation including temperature, salt concentration, light intensity, nitrogen source and CO2 concentration by Chlorella sp.. From RSM calculation, the theoretical equation Y＝273.2812－82.8X1＋19.458X2＋13.3347X3－66.059X12－15.925X1X2－14.052X22＋8.95X1X3－1.3X2X3－34.222X32 is found. It showed the maximum lipid concentration in theory can be achieved 316 mg L-1 at optimal conditions of 25℃, 20.57 g L-1 of NaCl, 12890 lux, 0.275 g L-1 of nitrogen supply and 1 vvm of air flow after 10 days incubation. In practically, a average value of 302 mg L-1 lipid produced was obtained which is similar to the theoretical value.
| Identifer | oai:union.ndltd.org:TW/098YZU05063115 |
| Date | January 2010 |
| Creators | Wen-Chieh Wu, 吳文傑 |
| Contributors | 藍祺偉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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