碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 98 / In this study, we isolated 88 strains including 84 Gram-negative bacteria and 4 Gram-positive bacteria, collected from a spring located inside Nature Valley, Hsinchu County, and seawater sample of the Nanjing fishing port, Hsinchu County, and seawater and coral from Kenting coast, Pingtung County, and shrimp paste. Phylogenetic analyses based on 16S rRNA gene sequences indicated that these strains belonged to twenty-two genera, including Hydrogenophaga、 Azonexus、 Sphingomonas、 Methyloversatilis、 Agrobacterium、 Aquabacterium、Alteromonas、 Pseudoalteromonas、 Marinomonas、 Erythrobacter、 Ruegeria、 Citromicrobium、 Flammeovirga、 Vibrio、 Curtobacterium、 Bacillus、 Paracoccus、 Photobacterium、 Lysobacter、 Pseudovibrio、 Microbulbifer、 Vibrio、 Thalassomonas.
A novel bacterial strain designated B3AT, isolated from shrimp paste was investigated by a polyphasic taxonomic approach. The cells stained Gram-positive and were aerobic, non-pigmented, sporulating and rod-shaped with a polar flagellum. The 16S rRNA gene sequence analysis indicated that strain B3AT belonged to the class Bacilli and was a member of the family Bacillaceae. Strain B3AT showed low level of 16S rRNA gene sequence similarity (＜94.0 %) with respect to any other genera in the family Bacillaceae and was most closely related to Halalkalibacillus halophilus BH2T (93.8 % sequence similarity). The isolate was able to grow at 20-45 °C, 0.5-15 % NaCl and pH 6-9. Menaquinone with seven isoprene units (MK-7) was the major respiratory quinone and iso-C16:0, anteiso-C15:0, and iso-C14:0 were the major fatty acids. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 45.3 mol%. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and physiological data, strain B3AT represents a new genus and species in the family Bacillaceae for which the name Allobacillus halotolerans gen. nov., sp. nov. is proposed. The type strain of Allobacillus halotolerans is B3AT (=BCRC 17939T =LMG 24826T).
A Gram-negative, non-pigmented, rod-shaped, facultatively aerobic bacterial strains, designated d8-1T ,was isolated from a freshwater spring sample. Optimum growth occurs at 35-40 °C, 0-1% NaCl and pH 7.0-8.0. The strains harboured the nitrogenase gene nifH, but nitrogen-fixing activities could not be detected in nitrogen-free culture media. Their closest neighbours were Azonexus caeni Slu-05T (96.7- 96.8 % similarity) and Azonexus fungiphilus BS5-8T (96.3- 96.6 %).The DNA–DNA relatedness of the novel strains to these two species of the genus Azonexus was less than 70 %. The isolates could also be differentiated from recognized members of the genus Azonexus on the basis of phenotypic and biochemical characteristics. It is evident, therefore, that the strain represent a novel species of the genus Azonexus, for which the name Azonexus hydrophilus sp. nov. is proposed. The type strain is d8-1T (=LMG 24005T =BCRC 17657T).
A short-rod-shaped, Gram-negative, motile bacterial strain, designated CS-6T, was isolated from a water sample collected from a spring located inside Nature Valley, Hsinchu County, Taiwan, and was characterized using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Aquabacterium in the class Betaproteobacteria. The closest neighbours were Aquabacterium parvum B6T (96.7% sequence similarity), Aquabacterium commune B8T (96.6 %) and Aquabacterium citratiphilum B4T (95.9 %). The predominant fatty acids were C18:17c (30.5 %), C16:0 (27.9 %) and summed feature 3 (C16:1 7c and/or iso-C15:0 2-OH) (22.7 %). The DNA–DNA relatedness of the strain with respect to recognized species of the genus Aquabacterium was less than 70 %. The isolate was also distinguishable from members of the genus Aquabacterium on the basis of phenotypic and biochemical characteristics. It is evident from the morphologic, chemotaxonomic and genotypic data, therefore, that strain CS-6T represents a novel species of the genus Aquabacterium, for which the name Aquabacterium fontiphilum sp. nov. is proposed. The type strain is CS-6T (= LMG 24215T = BCRC 17729T).
A bacterial strain designated KTW-12T was isolated from the reef-building coral in Kenting, Taiwan and was characterized using the polyphasic taxonomic approach. Strain KTW-12T was Gram negative, semitransparent, slightly curved rod-shaped, and non-motile. Growth occured at 15-35 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0), and with 0.5-6 % NaCl (optimum, 2 %). The major cellular fatty acids were summed feature 3 (C16:17c and/or C16:16c), C14:0 and C16:0. The DNA G+C content was 47.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain KTW-12T was most closely related to Vibrio porteresiae MSSRF30T with 94.8% gene sequence similarity. Further multilocus sequence analysis using rpoA, recA and pyrH genes also revealed low levels of sequence similarities (74.6-85.0 %) with all species of the genus Vibrio with validly recognized names. A multigene phylogenetic tree using concatenated sequences of the four genes (16S rRNA, rpoA, recA and pyrH) elucidated that strain KTW-12T occupied a distinct phylogenetic position, forming a long branching that was not clustered with any other known Vibrio species. On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, strain KTW-12T should be classified as representing a novel species, for which the name Vibrio stylophorae sp. nov. is proposed. The type strain is KTW-12T (= BCRC 80105T = LMG 25357T).
| Identifer | oai:union.ndltd.org:TW/098nkim8084003 |
| Date | January 2010 |
| Creators | Sing-Rong Jiang, 江幸蓉 |
| Contributors | Wen-Ming Chen, 陳文明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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