碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 98 / The purpose of this study is to establish the process conditions for preparing hydrolysate from pacific saury in order to provide information for developing its new product. Hydrolysates from pacific saury were obtained by using thermal process, acid treatment, and proteinase hydrolysis. Among them, proteinase showed the best efficiency in hydrolysis ratio, followed by acid treatment and thermal process.
The proteases of alcalase, bromelain, flavourzyme, papain, pepsin, protamex, protease-N and trypsin were used for preparing hydrolysate from pacific saury. Alcalase, flavourzyme, and protamex showed higher activity in hydrolysis of fish, and were selected in the following studies. The fish was then treated with two enzymes system including alcalase plus flavourzyme, protamex plus flavourzyme, and alcalse plus protamex. Alcalse plus flavourzyme showed the highest activity in hydrolysis ratio, and the optimal conditions were in the following: enzyme activity, 9000 U/mL for alcalase, 9000 U/mL for flavourzyme; temperature, 45℃; fish concentration, 20%. Under consideration for industrial processing in 20% concentration of fish treated with two enzymes system, the optimal concentration of alcalase was 7%; flavourzyme, 3%.
The hydrolysates obtained from pacific saury treated with the above two enzymes system were used to prepare fish sauce. The process conditions were in the following: fish concentration, 20%; alcalase, 7%; flavourzyme, 3%; hydrolysis conditions, 45℃, pH 8.0 for 48 hours; fermentation conditions, 10% of salt concentration, 10% of Aspergillus oryzae, 37℃, pH 6.0 for 1 month. After fermentation for one month, total nitrogen in fish sauce was 1.486 g/100 mL, and amino nitrogen, 0.534 g/100 mL.
| Identifer | oai:union.ndltd.org:TW/098nkim8084012 |
| Date | January 2010 |
| Creators | Tz-Yu Huang, 黃子瑜 |
| Contributors | Jen-Min Kuo, 郭建民 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
Page generated in 0.0013 seconds