碩士 / 嘉南藥理科技大學 / 生物科技系暨研究所 / 99 / In mammals, maternal antibodies can be transferred to the offspring via
placenta. In poultry, birds transmit maternal antibodies to their offspring by
depositing the antibodies in the egg. The Immunoglobulin Y is a type of
immunoglobulin which is the major antibody in avian. The use of chickens for
the production of polyclonal antibodies provides several advantages over other
methods of antibody production in mammals. In contrast to mammalian serum,
chicken IgY is low cost, high yield, without sacrificing the animals. In this study,
Edwardsiella tarda and Aeromonas hydrophila were used to immunize Leghorn
chicken to produced antigen specific IgY antibodies. These antibodies were
intended to be used as oral vaccine against pathogen infection. The titer of
anti-A.hydrophila chicken IgY could reach 625 at 6th week. Anti-E. tarda IgY
showed to be able to decrease the growth of E. tarda at the concentration of 20%.
Furthermore, IgY also inhanced the survival rate of the A. hydrophila infected
tilapias from 5% to 50% when kept feeding with 50% of egg powder mixed with
comercial fish feed for 3 days after challenge. The survival rate of the E.tarda
infected tilapias were also improved from 25% to 60% and 70% via feeding
with 30% and 50% of egg powder respectively.
| Identifer | oai:union.ndltd.org:TW/099CNUP5111021 |
| Date | January 2011 |
| Creators | Wei-Jen Chang, 張維仁 |
| Contributors | Kuo-Yu Shen, 沈國愉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
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