碩士 / 嘉南藥理科技大學 / 營養與保健科技研究所 / 99 / With 655,000 deaths in worldwide is due to colorectal carcinoma per year. Epidemiological studies suggest that ingesting a lot of dietary flavonoids with fruits and vegetables may prevent the incidene of colorectal carcinoma in humans. Based on our researches, ethanolic extracts from Morinda citrifolia leaf (LES), methanolic (PoM) or aqueous (PoW) extracts from Polygonum odoratum young shoots (Po) exhibited higher polyphenolic contents and revealed that these compounds played a part in antioxidative activities. LES, sub-fractions from LES (F I-V), PoM and PoW were used to study anti-cancer activity by acting through caspase-3 and modulating the expressions of NF-kappa B or AP-1 in colorectal carcinoma cells (HT-29), respectively. Results suggested that LES, F I, F III (rutin), PoM and PoW inhibited the proliferation of HT-29 and was associated with an apoptotic mechanism involving increasing in AP-1 expression and caspase-3 activation. Moreover, effects of F I on cell viability, genotoxicity, induction of apoptosis and cell cycle arrest were all similar to those of LES in HT-29. However, inhibition of HT-29 proliferation, and NF-kappa B expression as well as caspase-3 activation of PoW were better than PoM. Therefore, F I and PoW should be significant sources of anti-cancer activity. It was worth further research in purification and identification of active compounds, and deeply investigation for anti-cancer effects. Moreover, a clear understanding of the molecular mechanisms of action of F I and PoW are crucial in the valuation of these potent molecules as potential prophylactic and therapeutic agents.
| Identifer | oai:union.ndltd.org:TW/099CNUP5255008 |
| Date | January 2011 |
| Creators | Wei-wei Chen, 陳偉瑋 |
| Contributors | Shih-ying Chen, Tsui-pin Lin, 陳師瑩, 林翠品 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 115 |
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