碩士 / 嘉南藥理科技大學 / 化妝品科技研究所 / 99 / In this study, the first part is to analyse the antioxidant capacity of Phyllanthus amarus. The Phyllanthus amarus belongs to plant of an Euphorbiaceae, for common roadside small grass in Taiwan. In previous studies, the extrace from the plant of Euphorbiaceae were found to show significant antioxidation and protect a liver function. For understanding the ability of the antioxidizes of P. amarus and promoting the effect that the antioxidizes of extract, we use simple partition and column chromatography, look for having excellent extracting method of antioxidize effect. Using this condition select suitable extract method again to promote P. amarus extract. Extract layer application to add in cosmetics products.
This paper detect the acetone extract of P. amarus, the antioxidizes effect of chromatography layer of water and Ethylacetate partition layer and ethy lacetate layer. The paper result confirmed P. amarus partition of water layer, ethy lacetate layer and ethy lacetate rough segmentation layer of the 9, 10, 11, water layers have the effect of the free radicals of clearance DPPH of above 80%; the detect result of the rate of the ABTS•+ cation clearance, show P. amarus partition of water layer, ethy lacetate layer and ethy lacetate rough segmentation layer of the 9-14 layers reach the clearance rate of above 80%; The total polyphenol content detect a result, ethy lacetate rough segmentation layer of 10-12 have the highest total phenol content.
This paper result confirmed, with the science experiment and the data. Identify P. amarus of the concentration of the antioxygenation effect at in middle and high polarity layers, therefore immediately acquire the extract that the excellent antioxidizes effect by simple chromatography, exclude doesn''t have the impurities of effect, the extract of the high performance is added to cosmetics products, enhance its function, technique and the competition ability of market.
Secondly, we searched the chemical constituents of the Formosan soft coral Sarcophyton tenuispiculatum, and also assayed other analysis of biological activities. 95% Alcohol crude extracts of S. tenuispiculatum were found to show significant antioxidant capability against DPPH, chelating effect of Fe2+, superoxide anion and total antioxidant capacity. Furthermore ethyl acetate crude extract by the partition that were found to show siginificant activity against SCC25 (human oral squamous cell carcinoma) and HepG2 (human hepatoma).
Therefore, this study led to the isolation of three natural products 1-3, including a new compound 1, along with two know compounds.The structures of compounds 1-3 were established by detailed spectral data analysis (MS, 1D and 2D NMR) and by the comparison spectral data with those of the related known compounds. The relative configuration of metabolite 1 was further confirmed by X-ray single-crystal diffraction analysis.
In the cytotoxicity test, compound 1 showed significant activity toward the above A431 (human epidermoid carcinoma), SCC25 (human oral squamous cell carcinoma) and A375 (human amelanotic melanoma) Furthermore, compound 2 did not show a significant cytotoxicy against HepG2、Hep3B amd PLC/PRF/5 (human hepatoma).
| Identifer | oai:union.ndltd.org:TW/099CNUP5793018 |
| Date | January 2011 |
| Creators | Hui-Chun Lee, 李蕙君 |
| Contributors | Guey-Horng Wang, 王貴弘 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 185 |
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