碩士 / 中臺科技大學 / 食品科技研究所 / 99 / Abstract
The objective of this study was to investigate the production of chitinase by the immobilized cells of Aeromonas caviae NCHU1 coagulated with sodium alginate. The optimization of the cells immobilized with the sodium alginate-calcium system was studied by evaluation of the free cells’ number leaks to broth culture. The cells’ immobilized conditions were investigated including the concentration of sodium alginate(4.0~6.0 %), calcium lactate(2.0~3.0 %) and the soaking time (20~40 minutes) of the coagulated beads in the calcium lactate solution. The broth cultures(chitin contained) of coagulated beads of the A. caviae NCHU1 for the production of chitinase were evaluated by analysis of enzyme activity and were compared with that of non-immobilized cells. The results showed that the optimized condition for the immobilization were performed with sodium alginate 2.5 % , calcium lactate 3.0 % and the soaking time 30 minutes for the coagulated beads in the calcium lactate solution. The chitinase activities of the immobilized A. caviae NCHU1 was 0.531 U/ml at cultured time 24 hr higher than that of non-immobilized cells 0.462 U/ml. The results showed the potential of the chitinase production by the chitin-broth culture of immobilized A. caviae NCHU1 cells coagulated with sodium alginate-Calcium system. Further studies for the broth culture with continue process for commercial application are necessary and the above results might be applied for the advanced investigation.
| Identifer | oai:union.ndltd.org:TW/099CTC05252003 |
| Date | January 2011 |
| Creators | Sheng-Ru Lu, 呂昇儒 |
| Contributors | Yung-Chi Chang, 張永吉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 101 |
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