碩士 / 中原大學 / 化學工程研究所 / 99 / The three-dimensional structure of the electrospun scaffolds which can mimic the physical structure of the extracellar matrix (ECM), and it could support the environment for cell growth. Nature polymer-sodium alginate (SA) and synthetic polymer-polycaprolactone (PCL) with excellent biodegradability and biocompatibility were widely used as a biomimetic artificial scaffold in tissue engineering. In this study, we induced two types of polymer which were fabricated the SA/PCL composite fibers by electrospinning. The composite fibrous scaffold could not only support the cell growth, but also have the ability to accelerate the wound healing. Besides, SA fiber could absorb the exudates of wound, and keep a moist interface on the wound surface, hemostatic properties etc.
At first, we fabricated PCL and alginate fiber without bead by electrospinning. The effect of spinning conditions on the electrospinning fiber morphology were investigated, the spinning conditions including the cosolvent composition, molecular weight, concentration, polymer blending ratio, applied voltage and working distance. And the uniformity of fibers was improved with controlling the feed flow rate. Then the composite fibers were crosslinking to keep the structure integrity in an aqueous medium. The alginate/PCL composite fibers were characterized through tensile test and water uptake ability. The cellular biocompatibility and hemostatic property of the composite scaffold were evalutated by the in vitro cell culture and the clotting time test, respectively.
The results showed that the bead-free structure on alginate and PCL fibers were successfully fabricated under the optimal spinning conditions, and the uniformity of fibers were also improved with controlling the feed flow rate. The average fiber diameter of the alginate and the PCL fiber is 0.39±0.07 m and 1.38±0.19 m, respectively. Then the composite scaffold was successfully crosslinking through Ca2+ ions, and the fibrous structure will stability in the aqueous medium.
The results of tensile test were indicated that it was no significant difference between the PCL fibers and SA/PCL composite fibers. The ultimate tensile stress and tensile strain are ~1.7 MPa, 300% for both PCL and SA/PCL composite fibers, respectively. Inversely, the water uptake capacity of SA/PCL composite fibers was remarkably higher than PCL fibers which could facilitate the wound-healing by absorbing the exudates of wounds.
When seeding the human fibrosarcoma cells (HT1080) on the scaffolds, in vitro MTT assay results indicated the electrospun scaffolds have excellent biocompatibility and without cytotoxicity. In addition, the clotting time test with whole blood showed that SA/PCL composite fibers have the good coagulant property compared with PCL fibers, PCL film and TCPS as control. The cytocompatibility and blood compatibility analyses proved the SA/PCL composite fibers have the great potential to develop a novel biomaterial on wound dressing applications.
| Identifer | oai:union.ndltd.org:TW/099CYCU5063028 |
| Date | January 2011 |
| Creators | Li-Ping Huang, 黃莉萍 |
| Contributors | Kueir-Rarn Lee, Yung Chang, 李魁然, 張 雍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 119 |
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