碩士 / 逢甲大學 / 環境工程與科學所 / 99 / Many countries have been devoted to study biofuels for the substitution of fossil fuels. Studies were mainly concentrated and focused on studying the biodiesel and the oil-producing algae. The aim of this study is to find the optimal culture conditions of fresh water green algae (Chlorella protothecoides, C. vulgaris, Ourococcus sp., Scenedesmus obliquus, and S. dimorphus) in view of their possible utilization as novel feedstock for biodiesel production. At the same time, the Nile red method is used to quantify the lipid content in the oil-producing algae.
The results showed the optimal culture conditions for C. protothecoides, Ourococcus sp. and S. dimorphus were at an air flow rate of 1.0 L/L．min, light intensity of 95 μmol/m2s (7000 Lux) and a continuous light cycle (light: dark, 24:0, h). The optimal culture conditions for S. obliquus and C. vulgaris were similar to what mentioned above, the only difference for S. obliquus was the light intensity of 143 μmol/m2s (10500 Lux); and the air flow rate was 2.0 L/L．min for C. vulgaris. Under the optimal culture conditions, algae growth rate were 1.8 - 3.1 times fast than statically cultured, with 2.2 - 4.4 days of reduction in generation time, and an increase of 1.6 - 8.2 times biomass.After seven day-cultured in optimal condition, the lipid in algae ranged from 28.5% to 42.7% (w/w) by Nile red method and from 14.5% to 36.2% (w/w) by gravimetric method. These results revealed that neutral lipid content determined by Nile red method was higher than that by gravimetric method. Additionally, Nile red method was more efficient to perform than gravimetric method. Therefore, the gravimetric method was used to determine lipid content of five algae and S. dimorphus had the highest lipid yield, which may make it an ideal source of biodiesel.
| Identifer | oai:union.ndltd.org:TW/099FCU05515002 |
| Date | January 2011 |
| Creators | Yuan-Jung Chang, 張元榮 |
| Contributors | Tai-Lee Hu, 胡苔莉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 64 |
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