碩士 / 康寧大學 / 休閒資源暨綠色產業研究所 / 99 / Algae are not only nutritious, but also economically valuable. There is an abundance of algae in both sea waters and fresh waters. Currently algae are being developed mainly into bait, animal food and cosmetics. Algae with high lipids content are known as oleaginous algae. Recently there have been studies on using oleaginous algae as a source of biodiesel. This study aims to investigate the growth characteristics of oleaginous microalgae, including Nannochloropsis oculata, Tetraselmis chui, Isochrysis aff. galbana, Chaetoceros muelleri, Chlorella sp. and Botryococcus braunii, under different CO2 concentration (0.03% (air), 2%, 5%, and 10%) and room temperature (around 32 0C).
In this study, the optimal cultivating concentration for the above-mentioned algae was 2% CO2. At 2% CO2, among autotrophic microalgae, Nanochloropsis oculata has the highest growth rate, 0.0383 hr-1, while among heterotrophic microalgae, Chlorella sp. has the highest growth rate, 0.0321 hr-1. In general, except for Chaetoceros muelleri, the growth rate of autotrophic microalgae was higher than that of heterotrophic microalgae. The reason for Chaetoceros muelleri was that the cultivating temperature of this study, around 32 0C, was not within its optimal ranges, 250C-280C. Furthermore, at 2% and 5% CO2, the sequence for the growth rate of the above-mentioned microalgae was as follows: Nannochloropsis oculata > Chlorella sp. > Botryococcus braunii> Isochrysis aff. galbana > Tetraselmis chui > Chaetocros muelleri. In other words, the growth rate of Nanochloropsis oculta was the highest among the selected microalgae.
In addition, analyzing with photoluminescence emission spectra, the lipids content in the cell of Nanochloropsis oculta which cultivated at 2% CO2 was found to be 55.2%. After being extracted by the supercritical CO2 extraction method, in total 3 times, the average lipids composition for Nanochloropsis oculta was: 42.52±2.09 % of C14, 52.77±2.45 % of C16, 1.25±0.375% of C18, 3.28±0.22% of C18：1, and 0.17±0.05% of C18：2. Namely, major contents in the lipids of Nannochloropsis oculta were C14 and C16.
| Identifer | oai:union.ndltd.org:TW/099LU005148024 |
| Date | January 2011 |
| Creators | Hsin-Yu Wang, 王馨玉 |
| Contributors | Chun-Sheng Wu, 吳春生 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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