碩士 / 明志科技大學 / 生化工程研究所 / 99 / Free radical plays an important role in aging and diseases. Astaxanthin is strong antioxidant carotenoids. The antioxidant capacity of Astaxanthin is 10 time better than which of carotenoid, 550 time better than which of the natural vitamin E, 17 time better than which of the grape seed, 220 times better than which of the lutein, 150 times better than which of the Q10 and 7 times better than which of the lycopene. Astaxanthin is by far the strongest of antioxidants found in nature. It has become the hot topic in the research of the cosmetic and health food.
Extraction of aqueous two-phase system began in the 1950s. Two hydrophilic substances are mixed in a certain concentration and then the aqueous two-phase system was formed spontaneously. The extraction of two-phase system can be operated under mild conditions with large capacity, and the process could be scaled up easily. Currently, the aqueous two-phase systems have been widely used in separation and purification of natural compounds of traditional Chinese herbs. It has become the important method of biotechnology and extraction technology for anti-aging products, medicine and other fields.
In our research, astaxanthin was extracted from the Haematococcus pluvialis. The research purposes are using aqueous two-phase system to extracted astaxanthin. And to explore the distribution between two phase under different conditions. We can use polyethylene glycol, salt and water to form aqueous two-phase systems, by changing the weight percentage of PEG and salt, temperature and extraction time. We aimed to study the optimum condition of the extraction of the aqueous two-phase system.
The results showed that the aqueous two-phase system formed by the 30% (w/w) PEG (M.W. 8000) and 14% (w/w) K3PO4 has the highest extraction efficiency. We also found that astaxanthin was mainly distributed in the PEG phase (upper phase). The distribution coefficient increases with increasing the molecular weight of PEG. The extraction rate decreased with increasing the extraction time. The distribution coefficient increases with decreasing the temperature.
| Identifer | oai:union.ndltd.org:TW/099MIT00723036 |
| Date | January 2011 |
| Creators | Che-Yun Huang, 黃轍勻 |
| Contributors | Ciao-Song Wu, 吳喬松 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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