碩士 / 國立中興大學 / 化學工程學系所 / 99 / This study examined column elution fractionation and supercritical anti-solvent (SAS) process applied for purification and micronization of zeaxanthin from micro-algal Nannochloropsis Oculata. Crude extract containing 24.3 mg/gext zeaxanthin obtained from ultrasonic stirred extraction subjected to a pilot-scale column chromatography was successfully to yielding the product containing zeaxanthin with purity of 349.4 mg/g and recovery of 85%. Response surface methodology designed supercritical anti-solvent process with feed flow rates ranged from 0.1ml/min to 0.3ml/min and injection times ranged from 12min to 48min were investigated afterwards. Nanoparticles with mean sizes ranged from 211nm to 2567nm having high purity of 84.5% (wt) were obtained from the SAS process at suitable conditions. Anti-oxidative ability of the 2,2-diphenyl-1-picrylhydrazyl was also studied. This work provided a low cost and environmental friendly process to produce pharmaceutical grade algal zeaxanthin using supercritical fluid micronization.
| Identifer | oai:union.ndltd.org:TW/099NCHU5063038 |
| Date | January 2011 |
| Creators | Yueh-Cheng Cho, 卓岳正 |
| Contributors | 張傑明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 77 |
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