碩士 / 國立中興大學 / 化學工程學系所 / 99 / Trehalose is a kind of natural functional disaccharide. In this study an integrated process following the concomitant purification and immobilization of recombinant trehalose synthase from Picrophilus torridus for the production of trehalose was established. Eupergit C was selected as a carrier in this work. The purified recombinant P. torridus TSase (PTTS) showed that the optimum pH and temperature of 6.0 and 50 °C, respectively, were not alternated upon immobilization. Nevertheless, the immobilized activity did exhibit the higher tolerance of temperature. A higher residual activity of 59.5% was observed for the immobilized enzyme under 70 oC whereas it was 4.1% for free enzyme. The maximum conversion rate of maltose to trehalose by both free and immoblized enzyme was independent upon the substrate concentration under the optimum reaction conditions, in which the yield of approximately 60% for trehalose was obtained. The reusability of the immobilized enzyme in a batch reactor was also studied and we found that the immobilized enzyme maintained approximately 92.8% of residual activity after 24 cycles. While using the continuous packed-bed reactor, a maximum yield of 41.7% for trehalose has been reached from 100 mM maltose with a flow rate of 0.1 ml/min.
| Identifer | oai:union.ndltd.org:TW/099NCHU5063076 |
| Date | January 2011 |
| Creators | Tzu- Chun Cheng, 程子鈞 |
| Contributors | 林松池 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
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