碩士 / 國立中興大學 / 生物科技學研究所 / 99 / Pennatae diatom’s cell wall (frustule) used in this experiment is mainly composed of biosilica (SiO2). Because of its high mechanical strength, chemical structure and stability, acid resistance, biological compatibility, less susceptible to microbial decomposition and other conditions, the appropriate chemical modification, frustules can be developed for the purification of biological material. In this study, clear frustules using sulfuric acid to remove organic matter were obtained and analyzed to elucidate their structures and properties. The surfaces of frustules were rich in 3D structures (foramen) with an outer pore diameter ranging from 249 nm ~ 360 nm, and the 2-4 smaller inner pore ranging from 50 nm ~ 115 nm inside of the outer pore, which also has smallest pore size around 20 nm inside inner pore. Porosity increases the surface area in contact with objects and three-dimensional space, but also enhances the density of the surface modification of chemical groups. In addition, a reference indicates that the surface can emit a weak and wide range wavelength from 505 nm - 540 nm (excitation wavelength of 405 nm) effect by silanol functional groups (Si-OH) on frustules surface.
Infectious bursal disease virus vp2-441 subviral particle was used in this study to detect the adsorption characteristics of non-modified frustules. Results had shown an adsorption capacity with a maximum of 1.7 ± 0.95 x 10-11 mole of VP2-441 per mg of non-modified frustules. Chemical modification of frustule surface was performed with isocyanatopropyl triethoxysilane (IPS) and iminodiacetic acid (IDA) in order to immobilize copper ions. Copper ions measured by the average adsorption capacity of 191 ± 10 μmole of Cu2+ per ml of modified frustules. Characteristics of modified frutsules are also analyzed by SEM, EDS and FT-IR to compare the differences before and after modification. Experimental method in protein adsorption is calculated averaged 3.1 ± 0.037 x 10-9 mole of VP2-441 per ml of modified frustules. In this study, it confirmed the IPS-IDA-Cu modified frustules can enhance the adsorption capacity of about 28 times, that the modified frustules have potential to be developed into material of protein purification system.
| Identifer | oai:union.ndltd.org:TW/099NCHU5111028 |
| Date | January 2011 |
| Creators | Meng-Chuan Wu, 吳孟娟 |
| Contributors | Min-Ying Wang, 王敏盈 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 44 |
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