碩士 / 國立中興大學 / 材料科學與工程學系所 / 99 / In order to reduce the probility of osteomyelitis, the chitosan-vancomycin-gelatin-calcium phosphate (Chi-Van-Gel-CaP) composite is deposited on Ti6Al4V alloys in the study. The vancomycin drug loaded composite coatings are characterized by polarization tests, X-ray diffractometry (XRD), Field emission SEM (SEM/EDS) analysis, Fourier transform infrared spectroscopy (FTIR), UV-visible spectrometer, inhibition zone method and cell culture with MTT, Alkaline phosphatase activity (ALP) and osteocalcin assays for the biological evaluation including proliferation, differenliation, and mineration. The results indicate that the vancomycin drug loading is enhanced from 139.20 to 259.74 μg/cm2 by adding gelatin (Gel-Cap-Van/HA) into the previous Cap-Van/HA composite coating and farther enhanced to 435.06 μg/cm2 by the top layer covering of chitosan (Chi/Gel-Cap-Van/HA). At the same time, the drug release period increases from 7 to 30 days. Besides, the inhibition zone of Staphyl-ococcus aureus (S.A.) is 32 mm in diameter which can last more than 28 days. This means that the coating has effectively inhibited S.A. On the other hand, in vitro cell culture indicates that the adding of galatin may compensate the side effect of vancomycin on osteoblast, and the covering layer of chitosan can further improve the proliferation, differentiation and mineralization of osteoblast.
| Identifer | oai:union.ndltd.org:TW/099NCHU5159017 |
| Date | January 2011 |
| Creators | Chun-Yi Lo, 羅俊譯 |
| Contributors | 顏秀崗 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 51 |
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