碩士 / 國立中興大學 / 生物產業機電工程學系所 / 99 / The main propose of this study was to establish a rapid and accurate indicator of microalgae growth parameters such as microalgae cell size, cell division rate and absorption spectra. The cell size and cell division rate of five species (Chlorella sp., Nannochloropsis oculata, Isochrysis aff. galbana, Pavlova lutheri and Botrycocccus braunii ) were studied to correspond with microalgae growth phase and cell density, and to determine the feasibility of them as the assisted indicator for microalgae growth phase, lipid content, fatty acid content and composition. Moreover, the pH value, nitrate nitrogen, phosphate and absorption spectra (OD320-1000 nm) in the culture solution and the lipid content and composition of microalgae cells were also measured to inspect the correlation between each factor. Finally, the regression analysis was tested with the above date to expect that obtaining the indicators which is clear and easy measurement.
The experimental result obtained the high correlation between cell size, cell density and cell division rate of microalgae in each phase of growth stage, the correlation coefficients were all greater than 0.8. It demonstrate using microalgae cell size as an indicator for microalgae growth is suitable. Furthermore, microalgae can accumulate lipid and drive the synthesis of unsaturated fatty acid in the lag phase of growth stage; Microalgae cells growth rapid, Nutrients of culture solution have been absorbed quickly leading to changes in pH value and polyunsaturated fatty acids also showed a trend of reduction in the proliferative index phase; When entering the stagnation phase, there were different variations of fatty acid synthesis due to algal species.
Finally, the feasibility study of microalgae cell density, lipid content and fatty acid content forecast demonstrated that using two factors of microalgae characteristic wavelengths of absorbance and cell size, coefficient of determination (R2) were greater than 0.85 and P-Value were less than 0.05 of each factors of first-order regression equation was obtained, and the indicator for lipid synthesis was suitable. The results presented show the reference on cultivation of microalgae when harvesting in the best cell activity, lipid content and fatty acid composition.
| Identifer | oai:union.ndltd.org:TW/099NCHU5415020 |
| Date | January 2011 |
| Creators | Yu-Chien Kuo, 郭育謙 |
| Contributors | Chung-Chyi Yu, 尤瓊琦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 161 |
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