碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 99 / There were two aims in this study: (1) to confirm which type of thyroid hormone of tilapia was gifted from maternal origin; (2) when did thyroid hormone start to synthesize in tilapia larvae. We identified that 100 % of the yolk disappeared at 9 dph (day post hatching). We also observed whole body of T3 content was gradually lower before 11dph, but T4 contents can’t be detected at the same stage. Besides, TU can not inhibit T3 synthesis in 0- 13 dph, but inhibited effects by TU was observed during 13- 26 dph. According to the results, we suggested that the female fish transfer T3 to its filial generation, but not T4. On the other hand, the first thyroid follicular cells were found at 3 dph, and the immuno-cytochemistry (ICC) signals of TSH were located at pituitary of 9 dph tilapia. The T4 content was detected at 13 dph stage, and the type I deiodinase (D1) mRNA expression was started to increase at 15 dph. Based on these data, we confirmed that the maternal orgin provid T3 not T4 to the eggs. The thyroid hormone synthesis of tilapia was started at 9- 13 dph, and it showed a secretion peak on T4 at 19 dph.
| Identifer | oai:union.ndltd.org:TW/099NCYU5061001 |
| Date | January 2010 |
| Creators | Chih- Wei Hsu, 許值瑋 |
| Contributors | Su- Mei Wu, 吳淑美 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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