碩士 / 國立東華大學 / 海洋生物多樣性及演化研究所 / 99 / Benzophenone-3 (BP-3) is one of the most commonly used UV-filter compounds, as BP-3 absorb ultraviolet radiation. Recent findings have shown that BP-3 may have a xenoestrogen activity, which may negatively impact reproduction of aquatic organisms. The aim of this study was to investigate the potential effects of BP-3 on territorial behavior and gonad in male fighting fish (Betta splendens). Ten male fighting fish were exposed to solvent control (contain 0.1％EtOH), 100 ng/L 17α-ethinylestradiol (EE2), and 10 μg/L, 100 μg/L, and 1000 μg/L BP-3, respectively. The exposure period was 28 days. Standard length and body mass of the fish were measured at pre- and post- exposure for condition factor (CF). In addition, spontaneous swimming activity (total distance moved, activity level, absolute turn angle) and territorial behavior (maximum velocity, duration in attacking zone, and duration of opercular display) in front of a mirror were also analyzed. At the end of exposure, the fish gonads were sampled for gonadosomatic index (GSI) measurement and histology. Our results showed that the CF was significantly decreased after exposure to 100 ng/L EE2 and 1000 μg/L BP-3, but GSI was not significantly different between groups. Spontaneous swimming activity and duration in attacking zone were not significantly affected after exposure. However, fish in the 100 ng/L EE2 and 1000 μg/L BP-3 treatments showed significantly reduced maximum velocity and duration of opercular display. Relative proportion of mature spermatozoa in testicular tissue was significantly decreased in the 100 μg/L BP-3 treatments, but was not affected in the other groups. Collectively, the results show that BP-3 can disrupt territorial behavior of male fighting fish, suggesting an endocrine disrupting activity. This study also demonstrated that territorial behavior can be used as a behavioral biomarker in environmental toxicology testing.
| Identifer | oai:union.ndltd.org:TW/099NDHU5270029 |
| Date | January 2011 |
| Creators | Yea-Ting Wu, 吳雅婷 |
| Contributors | Te-Hao Chen, 陳德豪 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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