碩士 / 國立東華大學 / 海洋生物多樣性及演化研究所 / 99 / Organic UV-filters are used in a wide range of consumer products such as sunscreens and cosmetics. These compounds can enter the environment via various routes. Although estrogenic effects of these compounds have been previously described, their developmental and behavioural toxicity in fish have not been investigated. In this study, we examined the effects of two common UV-filter compounds and a paraben preservative on fish development and locomotor activity by a zebrafish embryo-larval bioassay. The zebrafish embryo were exposed to the UV-filters benzophenone-3 (BP-3) and octyl-methoxycinnamate (OMC) and a preservative butylparaben (BP) (0, 0.1, 1, 10, and 1000 mg/L) from blastula stage to 144 hour post fertilization (hpf). Survival, hatchability and abnormalities were monitored every 12 hr, and locomotor activity of the larvae was quantified at the end of exposure using an automated video-based animal movement tracking system. In addition, to assess cytotoxicity of these compounds, cell death and apoptosis levels were analyzed in the embryos at 72 hpf using acridine orange and TUNEL staining. Our data showed that (i) hatchability was completely inhibited in 10 and 1000 mg/L BP-3 and BP groups and was significantly decreased in 1 mg/L BP, but hatchability and larval survival were not significantly affected in all the other treatments; (ii) deformity rate was significantly increased only in the 1 mg/L BP-3 and BP groups; (iii) various changes of locotomor parameters such as moving distance, average or maximum velocity, and turn angle occurred in the exposed larvae; (iv) cytotoxicity was significantly increased in the 1 mg/L BP-3, as well as in the 0.1 and 1 mg/L BP groups. In conclusion, OMC seems to be less toxic than BP-3 and BP. It is noteworthy that the preservative butylparaben was equivalently or even more toxic than the UV-filters BP-3 and OMC. Our present study also demonstrated the value of fish locomotor analysis in assessing sublethal effects of emerging contaminants which display low acute toxicity.
| Identifer | oai:union.ndltd.org:TW/099NDHU5270030 |
| Date | January 2011 |
| Creators | Yen-Hsin Wang, 王彥心 |
| Contributors | Te-Hao Chen, 陳德豪 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 52 |
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