碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 99 / Histamine poisoning usually is associated with seafood such as tuna or sword fish containing high levels of histamine, which is generated by the decarboxylation of histidine. This reaction is caused by a bacterial enzyme, histidine decarboxylase. Therefore, inhibiting the growth of histamine producing bacteria (HPB) or the bacterial enzymatic reactions is a critical procedure to prevent histamine poisoning. Japanese horseradish (wasabi) is a popular spice in Taiwan and its major volatile compound, allyl isothiocyanate (AIT), has been reported to possess a high antibacterial activity. Therefore, tuna and sailfish samples were inoculated with HPB, Raoultella ornithinolytica and Morganella morganii, then placed in enclosed containers loaded with different concentrations of gaseous AIT at 15°C and 25°C. In addition, to simulate mishandling temperatures during storage, the samples were placed in low-high temperatures cycles such as 4°C-15°C-4°C or 4°C-25°C-25°C-4°C. Aerobic plate count (APC), HPB count, TVBN, and histamine concentrations were analyzed at certain periods. During study, histamine production increased at higher temperatures and sailfish producing more histamine than tuna. Samples inoculated with R. ornithinolytica produced higher amounts of histamine than the ones inoculaed with M. morganii. In the low-high temperature cycles, samples produced histamine at the high temperatures and continued producing histamine production at the low temperature, even though the samples were only exposed at the high temperature for a short period. Although the AIT treatments reduced only 1 to 2 log scale of APC and HPB population, a significant reduction (p＜0.05) of histamine in the fish samples was achieved. In addition, the higher reductions were obtained at a higher AIT concentration (p＜0.05). The inhibition mechanism could be that AIT retarded the enzymatic reaction or reduced production of histidine decarboxylase. In addition, the TVBN value were significantly reduced (p＜0.05) by applying gaesous AIT. Therefore, the results of this study demonstrated gaseous AIT could be an effective preservative and significantly inhibited the production of histamine in fish.
| Identifer | oai:union.ndltd.org:TW/099NKIMT084009 |
| Date | January 2011 |
| Creators | Yen, RuYu, 顏如玉 |
| Contributors | Lin, ChiaMin, 林家民 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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