碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 99 / In this study, we isolated 88 strains including 80 Gram-negative bacteria and 8 Gram-positive bacteria, collected from freshwater and seawater sample. Phylogenetic analyses based on 16S rRNA gene sequences indicated that these strains belonged to twenty-six genera, including Hydrogenophaga, Psedomonas, Bacillus, Catellibacterium, Rheinheimera, Pelomonas, Rhodoferax, Flectobacillus, Azospirillum, Delftia, Aeromonas, Curvibacter, Flavobacterium, Deinococcus, Acinetobacter, Flectobacillus, Acidovoraz, Limnobacter, Microbacterium, Agrobacterium, Duganella, Rubrivivax, Cellvibrio, Aquimarina, Stappia and Inhella. The results suggested that strains SPIO-19, TNR-25 and Ruye-53 might be the novel species, and they were subjected to further identification and classification. Strain antisso-27 exhibiting algicidal activity was subjected to algicidal activity analyses against cyanobacteria and green algae to evaluate the application of controlling algal blooms.
A light-yellow pigmented bacterial strain designated SPIO-19T was isolated from seawater in Taiwan and was characterized using the polyphasic taxonomy approach. Strain SPIO-19T was Gram-negative, obligately aerobic, rod-shaped, motile by means of one polar flagellum and non-spore forming. Growth occurred at 15-42 oC (optimum, 30-40 °C), at pH 5.0-9.0 (optimum, pH 8.0) and with 0.5-9.0 % NaCl (optimum, 1.0-5.0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SPIO-19T belonged to the genus Stappia and its closest neighbours were Stappia indica LMG 24625T and Stappia stellulata JCM 20692T with sequence similarities of 98.0 % and 97.9 %, respectively. Major fatty acid was C18:1 7c (>70 %). The major isoprenoid quinone was Q10 and the DNA G+C content was 64.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PMME), phosphatidylcholine (PC), an uncharacterized aminolipid and an uncharacterized phospholipid. The level of DNA-DNA relatedness of strain SPIO-19T to Stappia indica LMG 24625T and to Stappia stellulata JCM 20692T was 22.6 ± 2.8 % and 38.9 ± 3.8 %, respectively. On the basis of the genotypic and phenotypic data, strain SPIO-19T represents a new species in the genus Stappia, for which the name Stappia aquimarina sp. nov. is proposed. The type strain is SPIO-19T (= BCRC 80170T = LMG 25532T).
A yellow pigmented bacterial strain designated TNR-25T was isolated from spring water in Taiwan and was characterized using the polyphasic taxonomy approach. Strain TNR-25T was Gram-negative, obligately aerobic, rod-shaped, non-motile and non-spore forming. Growth occurred at 15-40 oC (optimum, 25 °C), at pH 6.0-10.0 (optimum, pH 7.0) and with 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TNR-25T belonged to the genus Inhella and its closest neighbour was Inhella inkyongensis IMCC1713T with sequence similarity of 98.1 %. The major fatty acids (＞10 %) of strain TNR-25T were summed feature 3 (comprising C16:1 7c and/or C16:1 6c) and C16:0. The major cellular hydroxy fatty acids were C10:0 3-OH and C12:0 3-OH. The isoprenoid quinone was Q8 and the DNA G+C content was 69.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylserine (PS), diphosphatidylglycerol (DPG) and several uncharacterized phospholipids. The DNA-DNA relatedness between strain TNR-25T and Inhella inkyongensis CCUG54308T was about 35.5 ± 1.8 %. On the basis of the genotypic and phenotypic data, strain TNR-25T represents a new species in the genus Inhella, for which the name Inhella fonticola sp. nov. is proposed. The type strain is TNR-25T (= BCRC 80211T = LMG 25721T).
A white pigmented bacterial strain designated Ruye-53T was isolated from culture pond in Taiwan and was characterized using the polyphasic taxonomy approach. Strain Ruye-53T was Gram-negative, obligately aerobic, rod-shaped, non-motile, non-spore forming and accumulation poly-β-hydroxybutyrate accumulating. Growth occurred at 10-37 oC (optimum, 20-25 °C), at pH 6.0-10.0 (optimum, pH 8.0-9.0) and with 0-1.0 % NaCl (optimum, 0-0.1 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Ruye-53T belonged to the genus Catellibacterium and its closest neighbour was Catellibacterium changlense JA139T with sequence similarity of 96.6 %. The major fatty acid of strain Ruye-53T was summed Summed feature 8 (comprising C18:1 6c and/or C18:1 7c；＞70 %). The isoprenoid quinone was Q-10 and the DNA G+C content was 61.2 mol%. The polar lipid profile consisted of a mixture of phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), several uncharacterized phospholipids and an uncharacterized aminolipid. On the basis of the genotypic and phenotypic data, strain Ruye-53T represents a new species in the genus Catellibacterium, for which the name Catellibacterium tilapiae sp. nov. is proposed. The type strain is Ruye-53T (=BCRC 80261T = KCTC 23310T).
In order to develop a biological agent for controlling the algal blooms, we isolated a bacterial strain antisso-27T that exhibited strong algicidal activity against the toxic Microcystis aeruginosa. Strain antisso-27T with brownish yellow pigmented was recently isolated from a water area of saltpan in Southern Taiwan. Phylogenetic analyses based on 16S rRNA gene sequences indicate that strain antisso-27T belongs to the genus Aquimarina in the family Flavobacteriacea and its only closest neighbor is Aquimarina spongiae (96.6 %). On the basis of the genotypic and phenotypic data, strain antisso-27T represents a new species in the genus Aquimarina, for which the name Aquimarina salinaria sp. nov. is proposed. In this study, algicidal activity analyses suggested that strain antisso-27T employed direct or indirect mode of algicidal lysis to Cyanothece sp. SC-2, Synechococcus sp. DJN-01, Chlamydomonas raudensis L4-2 and Chlorella pyrenoidosa FIG-14, nevertheless it released an algicidal substance against Microcystis aeruginosa MTY01.
| Identifer | oai:union.ndltd.org:TW/099NKIMT270002 |
| Date | January 2011 |
| Creators | Sheu, Fusian, 許復先 |
| Contributors | Sheu, Shihyi, 許世宜 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 166 |
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