碩士 / 國立澎湖科技大學 / 食品科學研究所 / 99 / In this study, 156 bacteria have been isolated from soil and decayed
brown algae.
Strain P44 showed the strongest alginate lyase activity among
isolates which can use alginate as sole carbon source. After gram staining,
spore staining. catalase test, API 50CHB identification and 16S rRNA
sequencing, P44 identified as Bacillus megaterium.
Bacillus megaterium P44 can break down poy-1,4-D-mannuronate
and poly-1,4-L-guluronate in the alginate polymer, produce protease and
catalase, and cannot use CMC and agar as carbon source.
After incubation at 37℃, 120 rpm for 72 to 86 hours in the enzyme
production medium, Bacillus megaterium P44 is able to produce alginate
lyase of 386.4 U / ml, the enzyme optimum temperature and pH values
are 45℃, pH5.5 ~ 6.5, and the optimal stability range should be 35℃, pH
5.5 to 6.
Bacillus megaterium P44 can produce alginate lyase 29 U / ml in
Sargasso medium which use 2% Sargasso powder as the sole carbon
source.
| Identifer | oai:union.ndltd.org:TW/099NPHT7252002 |
| Date | January 2011 |
| Creators | Hsueh, Chiuchin, 薛秋琴 |
| Contributors | Hu, Hunghsi, 胡宏熙 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 152 |
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