The Bioimformatic study of unique functional gene cloned from tilapia, Oreochromis mossambicus / 吳郭魚單一功能性基因的生物資訊研析

碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 99 / The expressed sequence tags, derived from the developing tilapia brain, were established in our laboratory. An unique gene, high mobility group proteins 2 (HMG2), were investigated. HMG2 is a non-histone chromatin protein. HMG2 cloned from tilapia, Oreochromis mossambicus, is a gene with open reading frame 642bps, and deduced as 213 amino acid protein sequence. NCBI Conserved Domain search, the Gene Ontology, NEBcutter restrict enzyme analysis, NCBI blastx, and neighbor phylogenetic tree were used for the bioinformatic analysis. In the present study, the protein expression of HMG2 in the BL21 E.coli system, a prokaryotic system, and purified with 6X His Tag NI-NTA affinity chromatography.

Identiferoai:union.ndltd.org:TW/099NSYS5270041
Date January 2011
CreatorsPin-Chin Huang, 黃品欽
ContributorsChing-Lin Tsai, 蔡錦玲
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format50

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