碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 99 / Functional Complex Beneficial Microorganisms (FCBM) is not only be classified as photosynthetic bacteria, nitrifying bacteria, and some probiotics, but also includes other microorganisms, such as Trichoderma and Monascus purpureus. In aquaculture, CBM can be used in different way, such in water processing, disease prevention, feed potentiation, and nutrient regulation. Nowadays, people are try to substitute expensive animal protein feed with cheaper plant protein in feed used in aquaculture. Plant-desired protein feed, however, contains fewer nutrients and more toxic compounds, when compared with animal protein feed. By conducting anaerobic solid fermentation of isothiocyanate (ITC), oxazolidinethione, (OZT), and phytic acid, which are remove from canola meal, CBM also can increase the plant-desired protein utilization in Oreochromis niloticus. In this study ,the optimal condition for the solid state fermentation of CBM was deterimented. CBM and mixed canola meal with a ratio of 0.8:1, 1:1, and 1.2:1 and subsequently fermention for 7, 14, 21, 28, 35 days and 5, 10, 15, 20, 25, 30 days, respectively. The results showed that the CBM mixture with canola meal on the ratio 0.8:1 has the lowest in toxic compounds and anti-nutritional factors. The result of canola meal was fermented under 25°C, 30°C, 35°C, 40°C, for 5, 10, 15, 20, 25, 30 days indicated that detoxification was most effectively at 30°C. The fermentation duration was 25 days detoxification with most effectively. Small peptides increased 10-fold when fermented for 25 days. The amount of isothiocyanate, oxazolidinethione, and phytic acid decreased 68%, 75%, and 81%, respectively and after 25 days fermentation. Afterwards, we used canola meal fermented and unfermented canola meal for 25 days to substitute 30%, 60%, and 100% soy meal substitute soy meal of 30%, 60%, and 100% for ten weeks feeding. The result showed that no significant difference between using 60% canola meal and control group after 10 weeks. In addition to, unfermented canola meal to substitute soybean meal 30% at most, and liver enlarged,vacuoles and necrosis can be observed in the liver biopsy. Furthermore, we used fermented canola meal and soy meal to substitute fish meal of 10%, 30%, and 50%. The result revealed that 10% canola meal has better growth performene than the group with no substitution. Although Oreochromis niloticu did not grow well, the liver biopsy was normal, or the liver enlarged,vacuoles and necrosis was reduced by using canola meal of 50%. This result suggested that the nutrients of canola meal increased and the toxic compounds and anti-nutritional factors were reduced significantly after fermented with CBM.
| Identifer | oai:union.ndltd.org:TW/099NTOU5086103 |
| Date | January 2011 |
| Creators | Hung-Ming Tsai, 蔡宏明 |
| Contributors | Jenn-Kan Lu, 陸振岡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 102 |
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