碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / In tissue engineering, growth factors and cells are important elements for repairing nerve injuries. Supplemented with exogenous growth factors is an effective medical strategy for protecting nerve injury and promoting nerve regeneration. It has been demonstrated that basic fibroblast growth factors have great effect on angiogenesis and neuronal cell survival; however, bFGF has two shortages: short half-life and easily degraded by enzymes. Therefore, the aim of this study is to design a biodegradable chitosan/fucoidan (C/F) nanoparticle to carry bFGFs and to maintain their activities. Furthermore, apply the bFGFs released from nanoparticles to promote nerve regeneration. According to our experimental results, chitosan and fucoidan formed stable nanoparticles by electrostatic interactions with nanoparticles size at 60~600 nm. The concentration of fucoidan affected the characteristics of C/F nanoparticles. At 0.5% (w/v) fucoidan solution, the prepared C/F nanoparticles not only protected bFGF from deactivation by temperature and enzymes but also controlled the release of bFGFs. By in vitro cell studies, there was no cytotoxicity observed on PC12 cells. The differentiation of PC12 cells were promoted by released bFGF in the early stage. Briefly, C/F nanoparticles are potential carriers for delivery bFGFs. The effect of bFGFs released from C/F nanoparticles on axonal elongation and animal experiments will be studied as a preclinical trial before clinical application in the near future.
| Identifer | oai:union.ndltd.org:TW/099NTOU5253038 |
| Date | January 2011 |
| Creators | Ya-Ting Yang, 楊雅婷 |
| Contributors | Yi-Cheng Huang, 黃意真 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 99 |
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