碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / Gymnodinium catenatum is dinoflagellates formed chain-likes colony, which could produce paralytic shellfish poison (PSP). The aquatic animals which filter the foods will accumulate PSP in their bodies, and humans may be poisoned by the way of food chain. G. catenatum was discoveried in Maxico by Graham in 1943, and the food poisoning incidents appeared in Argentina, Uruguay, Australia, Morocco, Spain, Japan, and Philippines during recent twenty years. In recent years, China and Hong Kung also found G. catenatum. In Taiwan, the PSP incidents occurred in 1986 in Pingtung and in 1991 in Chaiyi, and the toxins were both identified came from the toxic dinoflagellate Alexandrium minutum. Some of toxic dinoflagellates could be affected by the environmental nutrients. Nitrogen is one of the important factors because there was 33% of nitrogen in PSP. In the first part of this study, we used NaNO3, NaNO2, NH4Cl, and CH4N2O with different concentrations (7.5 ppm, 75 ppm, 750 ppm of N) to culture G. catenatum, and compared with their situations of growth and toxin. The results showed that high concentration (750 ppm) of nitrite made G. catenatum grow slowly, and urea made the growth best in the low concentration (7.5 ppm). The total toxicity of G. catenatum did not have significant variations of these four nitrogen sources, but the single cell toxicity was decreased during the culture time. The composition of algal toxin was C1, C2 and saxitoxin (STX), and STX was more than 50%. The components and percentages of toxin would not vary significantly with different nitrogen sources.
Liquid culture and slant culture were the two primary treatments for microalgae in preservation laboratory. Some studies reported that the toxic alga might have some variations, for example: loss of toxicity, when cultured at artificial environment in laboratory for a long time. In the second part of this study, we found that whether A. minutum and G. catenatum would be different in growth, toxicity, and composition after treatment with immobilization. The results showed that growth curve of A. minutum was not changed, but the crude protein was significant increased, and the toxicity was higher than that of non-immobilization. G. catenatum could not grow successfully after treated with immobilization. The possible reason was that the individuals of G. catenatum were hurt by treatment of centrifugation.
| Identifer | oai:union.ndltd.org:TW/099NTOU5253059 |
| Date | January 2011 |
| Creators | Wei-Cheng Lin, 林威成 |
| Contributors | Deng-Fwu Hwang, Yean-Chang Chen, 黃登福, 陳衍昌 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 94 |
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