碩士 / 國立臺灣海洋大學 / 食品科學系 / 99 / Colorectal cancer (CRC) is one of the most common gastrointestinal tract malignancies worldwide, principally in the western and westernized countries. Patients with inflammatory bowel disease (IBD), both ulcerative colitis (UC) and Crohn’s colitis, are at increased risk of developing colorectal cancer (CRC). intestinal inflammation (ulcerative colitis and Crohn’s disease) also drastically increases the risk of developing colon cancer and the degree of inflammation correlates with cancer risk. IBD is a widespread and debilitating disease characterized by inflammation and immune cell infiltration and immune- mediated destruction of the gastrointestinal tract. Many studies have confirmed that brown algae extracts have antioxidant and anti-inflammatory properties, especially in fucoxanthin. However, anti-inflammatory effects of fucoxanthin in IBD have not been investigated. This study that we use fucoxanthin brown algae extract (BAE) from Sargassum glaucescens was assessed via inhibitory effect of nitric oxide (NO) production in lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells. Moreover, the release of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and the mRNA expression levels of interleukin-1β (IL-1β) and cyclooxygenase-2 (COX-2) were reduced by the addition of fucoxanthin in a dose-dependent manner. IBD model was induced by administration of dextran sulfate sodium (DSS) (5% W/V) in drinking water to male C57BL/6 mice for seven consecutive days and estimated disease activity index (DAI). DSS produced shrinkage of colon length and increased the relative colon weight/length ratio accompanied by mucosal edema and bloody stool. In IBD model we were oral administration of BAE for seven consecutive days after DSS challenge. In the colitis-associated colorectal cancer (CAC) model, mice were treated with DSS for acute colitis and azoxymethane (AOM), BAE was tested in prevention of colitis-associated colorectal cancer in the AOM-DSS model. BAE mitigated the injurious effects of DSS and ameliorated all the altered biochemical parameters. These results suggest that fucoxanthin brown algae extract could possibly have improve injurious effects of DSS and that it may have therapeutic value in the setting of IBD and CAC.
| Identifer | oai:union.ndltd.org:TW/099NTOU5253075 |
| Date | January 2011 |
| Creators | Jia-yuan Hu, 胡嘉元 |
| Contributors | Zwe-Ling Kong, 龔瑞林 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 116 |
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