碩士 / 國立臺灣大學 / 食品科技研究所 / 99 / Alzheimer’s disease is the most common form of dementia. It is a neurodegenerative disorder characterized by impairment in cognitive functions and changing in mood and behavior. The hallmarks of this disease are the presence of extracellular beta-amyloid deposition and intracellular hyperphosphorylated tau protein. Senescence-accelerated mouse prone-8 (SAMP8) is an age-accelerated mice model rather than gene-mutated mice model. In addition, SAMP8 mice also show the cognitive impairment and beta amyloid deposition. The objectives of this study were to investigate the potentials of sesamol and Z-ligustilide to ameliorate learning / memory impairment and Alzheimer’s disease pathophysiology in SAMP8 mice. Our results demonstrated that a 12-week oral administration of Z-ligustilide or sesamol could consolidate the fear-conditioning memory of SAMP8 mice and also slightly improved the spatial learning and memory. Using immunohistochemical (IHC) staining for certain brain sections of mice, we found that there was a decreasing trend in the deposition of beta amyloid after administration of sesamol and Z-ligustilide. Using ELISA to detect beta amyloid, the results were similar to IHC staining; however the treatment groups were not significantly different from the control group. To evaluate the oxidative stress status of the mice, liver TBARS were determined. We found that treatment groups had lower TBARS level. To correlate the results of behavior tests, beta amyloid content, and oxidative stress status, we concluded that there was neither strong correlation between cognitive impairment and beta amyloid, nor oxidative stress and beta amyloid. These results suggested that beta amyloid and oxidative stress may not be the crucial factors to influence cognitive ability in SAMP8 mice.
| Identifer | oai:union.ndltd.org:TW/099NTU05252013 |
| Date | January 2011 |
| Creators | Po-En Chiu, 邱博恩 |
| Contributors | 孫璐西 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 213 |
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