碩士 / 國立臺灣大學 / 海洋研究所 / 99 / Recently, the corals in Lyudao, Taiwan, are prevalently suffered from the attack of “Black Disease”, caused by the overlaying of an encrusting sponge T. hoshinota. To understand the insights underlying T. hoshinota’s behavior, we sampled different parts of the sponge in Lyudao, including front-end, middle-site, and an isolated culture in mesh box as a control, for comparative purpose. The morphology of T. hoshinota was studied using electron microscopes, and the transcriptome profile was analyzed for validating possible associations between gene expression and appearance. The electron microscopes observation indicated that there were many coccus-like cyanobacteria, and tylostyle spicules were arranged regularly in the mesh box sample but disorderly in the front-end sample. Also, the front-end sample was in a healthier condition as being more intact compared to other samples. According to the transcriptomic analysis, the front-end sample had higher expression of the energy-related genes, suggesting that the front T. hoshinota might acquire energy by using coral as “food resource” for proliferation and territory expansion. The middle-site sample expressed more transcripts related to apoptosis and DNA repair, saying that this part of the sponge might be under stress. The preferred expression on cell motility genes in the mesh box sample revealed possible purpose of its silk-like appearance and might lead T. hoshinota to move. This transcriptomic study is just a start uncovering physiological and biochemical features of T. hoshinota. Further understandings about the interactions between coral, sponge, and associated bacteria can be obtained when the microbial gene expression data were incorporated.
| Identifer | oai:union.ndltd.org:TW/099NTU05279031 |
| Date | January 2011 |
| Creators | Cheng-Yu Yang, 楊承諭 |
| Contributors | Sen-Lin Tang, 湯森林, 謝文陽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 103 |
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