碩士 / 國立臺灣大學 / 動物學研究所 / 99 / Grouper survivors after nervous necrosis virus (NNV) acute infection will become persistently infected. In order to reveal the relationship of grouper Mx protein and the persistent infection of NNV, a cell line GB was established from the brain tissue of grouper survivors. NNV was found in GB cells by RT-PCR, and NNV protein was only detectable in the cytoplasm of a few GB cells by immunohistochemistry staining, suggesting that only a few cells were acutely infected and most cells were likely protected. After serial treatments of NNV-specific polyclonal antibodies, GB cells became a NNV-free cell line which was named as cured GB (cGB). The expression of grouper Mx (GMx), a downstream protein of interferon response, was detected in GB cells, NNV-infected cGB cells and poly I:C-transfected cells, but was abscent in cGB cells, indicating that IFN response existed in GB cells, and IFN response and Mx expression was inducible in cGB cells by NNV infection and poly I:C transfection. When cGB cells were infected by NNV with low MOI, all characteristics of persistent infection in GB cells reappeared in infected cGB cells. In addition, NNV titer proliferated in poly I:C-transfected cGB cells were lower than that in cGB cells, indicating that the mechansim of NNV persistence in GB cells possibly mediated through IFN and Mx protein. By the way, NNV. At 24 h post-infection, only viral RNA-dependent RNA polymerase (RdRp) was found to be colocalized with Mx at perinuclear area, suggesting that Mx might interfere with NNV replication by interaction with RdRp.
| Identifer | oai:union.ndltd.org:TW/099NTU05312007 |
| Date | January 2011 |
| Creators | Ju-Chien Chan, 詹茹潔 |
| Contributors | Shau-Chi Chi, 齊肖琪 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 58 |
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