碩士 / 國立臺灣大學 / 動物學研究所 / 99 / Synaptic vesicles (SVs) store neurotransmitters and are transported to axon terminals for synaptic transmission. We are interested in how the vesicle movement and synaptic transmission are modulated by the cytoskeleton. To monitor the movement of SVs, synaptophysin was fused with photoactivatable green fluorescent protein (PA-GFP) and expressed in cultured cortical neurons. Synaptophysin-PA-GFP was located at some spots and moved slowly in neuritis captured by CCD camera. To monitor the synaptic transmission, UV flash was applied at designated regions repetitively to photolyze MNI-caged-glutamate to stimulate the target cell and the elevations in cytosolic Ca2+ concentration were visualized by fluo-2 MA. The vesicle movement was slowed down from ~0.7 μm/s in control group to ~0.5 μm/s in neurons treated with cytoskeleton antagonists; the Ca2+ responses in both target and connected neighbor cells were reduced as well. When KIF17, a kinesin for SV transportation, was expressed in neurons, the vesicle movement was significantly increased to ~0.8 μm/s. Calmodulin and its mutants interfered vesicle movement and neurotransmission but without significance. After being treated with DHA, the Ca2+ elevations in target cells were enhanced. These data suggest that slowing down SV movement may result in modulate the synaptic transmission and synaptic plasticity.
| Identifer | oai:union.ndltd.org:TW/099NTU05312016 |
| Date | January 2011 |
| Creators | Chin Lin, 林靖 |
| Contributors | 潘建源 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 65 |
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