碩士 / 國立臺灣大學 / 微生物學研究所 / 99 / Previous study showed that Myb2 and Myb3 transcription factors may regulate inducible transcription of an adhesion protein, ap65-1, gene in the protozoan parasite Trichomonas vaginalis, and external stimuli, such as iron and H2O2 , may affect entry of these proteins into the nucleus, presumably to regulate downstream gene expression. The purpose of this study was to clarify whether Myb2 or Myb3 may complex with other proteins in the cytoplasm.
Using the gel filtration and Western blot assay, the data showed that Myb2 and Myb3 could form complexes in the cytoplasm. Myb2 and Myb3 were mainly localized to the nucleus by an immuneflouorescence assay (IFA), suggesting that they may be actively translocated into the nucleus. I explored a cross-linking coupled immunoprecipitation (IP) approach to purify Myb-interacting proteins, and used liquid chromatography-tandem mass spectrometry for protein identification. Although the protocol needs to be further refined, this work may provide tool for further investigation the roles of Myb proteins on inducible-gene regulation.
| Identifer | oai:union.ndltd.org:TW/099NTU05381140 |
| Date | January 2011 |
| Creators | Chung-Chi Kuo, 郭純綺 |
| Contributors | Shiou-Jeng Ong, 翁秀貞 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
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