碩士 / 國立臺灣科技大學 / 材料科學與工程系 / 99 / In this study, alginate beads are used to be the carrier of calcium ions and delay the gelation of alginate. In order to facilitate the growth of cells, collagen was added into the alginate solution. PluronicR F127(F127) was also mixed with alginate solution to make a thermosensitive scaffold. The mechanical properties were measured by DMA and Rheometer, which displayed the thermosensitivity of gel and the modulus of gelation before and after. The evenness of calcium ions in gel was proved by UV-Vis measurements for calcium ions release in alizarin red S (ARS) solution. The distribution of calcium ions in alginate gel after dying by ARS solution was also observed by optical microscope. For biocompatibility, L929 fibroblasts and hFOB 1.19 osteoblasts were tested in this study. The cell toxicity test was done by MTT assay and cell adhesion test was proved by Trypan Blue staining. The results showed that the gelation occurred in a gradual manner by the change of modulus since the release of calcium ions increasing by time and cause ionic crosslinking which update the elasticity of gel. Thermosensitivity in gel also appeared under 37℃ because of adding F127. In addition, the growth of L929 fibroblasts and hFOB 1.19 osteoblasts evaluated non-toxicity in gel. The results from cell adhesion test also proved L929 fibroblasts and hFOB 1.19 osteoblasts can attach the surface of scaffold and grow. These results showed that this scaffold have possibility to applied in bone materials.
| Identifer | oai:union.ndltd.org:TW/099NTUS5159027 |
| Date | January 2011 |
| Creators | Ting-Yuan Chen, 陳婷媛 |
| Contributors | Ming-Chien Yang, 楊銘乾 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 58 |
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