碩士 / 國立臺灣科技大學 / 化學工程系 / 99 / Borzi found the cyanophycin by the observation of blue-green algae. Its appearance is granular and it is composed of the equimolar ratio aspartic acid and arginine. The backbone of cyanophycin is aspartic acid, and the side chain structure is composed arginine.
In this study, we used E.coli which include Synechocystis sp. PCC6803 cyanobacteria protein synthase cphA to produce cyanophycin. Then the produced cyanophycin were used for physical and biocompatibility assays. We used FTIR to analyze the functional groups of cyanophycin, SEM to observe the surface which is cross linked by glutaraldehyde and cyanophycin, TGA and DSC to detect the thermal stability and melting point, and assay the protein adsorption.
The results showed of protein adsorption experiment confirm no protein adsorption. The melting and recomposition points of soluble and insoluble cyanophycin are about 170~185 oC and 250 oC, respectively.
In biocompatibility, we test the biological immune response and cell adhesion. The Raw 264.7 culture on the surface of insoluble cyanophycin cross-linked with glutaraldehyde and of soluble cyanophycin medium, and compare the toxicity with LPS stimulus. The final result, the insoluble cyanophycin cross-linked and sluble cyanophycin didn’t provoke the immune response. For CHO cell on the insoluble cross-linked surface and high concentration medium, the relative grow velocity is reduce, but for Raw 264.7 is near control.
Summary, cyanophycin did’t provoke immune response, has good thermal stability, contain lots of amino group which can react easily for modification the characterization.
| Identifer | oai:union.ndltd.org:TW/099NTUS5342026 |
| Date | January 2011 |
| Creators | Shih-chung Huang, 黃世昌 |
| Contributors | Wen-chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
Page generated in 0.0019 seconds