碩士 / 國立臺灣科技大學 / 化學工程系 / 99 / Bacterial cellulose has recently attracted a great deal of attention for biomedical applications because of its good water retention, mechanical properties, and biocompatibility. However, the lack of suitable functional groups provides a poor surface for cell growth. Soluble cyanophycin is non-ribosomal protein which is composed of aspartic acid, arginine, and lysine. In order to improve cell adhension and growth on bacterial cellulose, we added water soluble cyanophycin to the growth medium of Acetobacter xylinum in an attempt to produce bacterial cellulose/soluble cyanophycin composites. The physical properties of modified bacterial cellulose were analyzed. The results showed that the addition of cyanophycin into the cultivation of Acetobacter xylinum caused aggregation and crystallization of glucan chains into cellulose microfibrils and ribbons, resulting in thicker bundles or sheet fibrils. It also resulted in the decreases in the mechanical property and crystallization. The analysis showed that water soluble cyanophycin formed a composite with bacterial cellulose. We use different types of cells: Chinese hamster ovary cells, porcine chondrocytes, human umbilical vein endothelial cells, respectively, to the cell growth on the composites examine.
Compared to the unmodifield bacterial cellulose, cellular viability was higher than 30% for all tested samples by MTT assay. As a result, the bacterial cellulose/water soluble cyanophycin composites could improve cell attachment and proliferation .
| Identifer | oai:union.ndltd.org:TW/099NTUS5342027 |
| Date | January 2011 |
| Creators | Po-shao Chen, 陳柏劭 |
| Contributors | Wen-chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 113 |
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