碩士 / 國立陽明大學 / 環境與職業衛生研究所 / 99 / Until the last decade, little attention was given by the neuroscience community to the neurometabolism of metals, since it has been linked to major neurodegenerative diseases. Human exposure to toxic metals or the homeostasis of essential trace elements altered in human may cause neurosystemic disorders or neurodegenerative disease, such as Alzheimer's disease, Parkinson's disease or attention deficit hyperactivity disorder. However, there are limited data and information on the plasma metal levels in patient of AD, PD and ADHD in Taiwan.
The aims of this study are to compare the plasma metal concentrations between neuropsychological patients and general population, and to predict the risk factors of metal exposure. Patients including Parkinson's disease (PD), Alzheimer's disease (AD), and attention-deficit hyperactivity disorder (ADHD) were invided to join this study. A total of 156 plasma samples were collected from patients with AD (n=30), PD (n=30), ADHD (n=26) and healthy subjects (n=60). Samples were collected after approved by the Institutional Review Boards of Taipei Veterans General Hospital and of Chang Gung Memorial Hospital. Written informed consent was obtained from each subject. The mercury concentration was determined by mercury analysis system and other metal concentrations (As, Pb, Fe, Co, Ni, Cu, Zn, Mg, Na, K and Ca) were determined by ICP-MS.
Results of this study indicate that the concentrations of AD, PD, ADHD and healthy subjects are significantly differet. The univariate analysis showed that plasma metal concentrations of the general population are affected by gender, occupation and dietary habits (such as vegetarian and frequency of seafood and meat intake).
Regression analysis indicates that (1) the higher As, Mg, Fe, Co, Zn, K, Ca levels and lower Na levels in AD patients compared with healthy subjects (p-value <0.01), (2) the higher Hg, Na levels and lower As, Pb, Mg, Fe, Co, Ni, Cu, Zn, K, Ca levels in PD patients compared with healthy subjects (p-value <0.005), (3) the higher Hg, As, Mg, Co, Ni, Cu, Zn, K, Ca levels and lower Na levels in ADHD children compared with healthy subjects (p-value <0.005), (4) the lower Pb, Co and Cu levels in patients with AD compared with PD (p-value 0.008~0.033).
In a word, plasma metal concentrations among the patients of AD, PD and ADHD are significantly different. Comparing to healthy subjects , the concentrations for the patients are also significantly different. This study also investigate the relationship of metal concentration between plasma and whole blood. The result shows that a significantly positive correlation for mercury (r = 0.726), arsenic (r = 0.439), iron (r = 0.381), cobalt (r = 0.399), Ni (r = 0.388), copper (r = 0.787), zinc (r = 0.574) and a significantly negative correlation for potassium (r = -0.491). Therefor, the plasma might serve as appropriate alternative of whole blood for the determination of Hg, As, Fe, Co, Ni, Cu and Zn concentrations.
| Identifer | oai:union.ndltd.org:TW/099YM005515006 |
| Date | January 2011 |
| Creators | Yu-Zhen Zheng, 鄭于甄 |
| Contributors | Mei-Lien Chen, 陳美蓮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 132 |
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