碩士 / 元智大學 / 生物科技與工程研究所 / 99 / Polyhydroxyalkanoates (PHAs), a biodegradable polymer, by the different chemical structure on the length of the main chain and side chain composition, can be developed over 120 different types and categories. PHAs are natural polyester, mainly for the growth process of bacteria, due to excess carbon and other nutrient sources (such as nitrogen and phosphorus) deficiency, bacteria will accumulate PHAs by exceeding carbon metabolism. PHAs have the properties of plastic and fossil fuel depletion, to replace the existing petrochemical plastic products with the potential for development. However, PHAs’ application on industrialization is still confined to its high production costs. Therefore, reducing PHAs production costs will be an important issue for its commercialization process in the near future. Thus, this study aims to develop a chip manufacturing process for the production of PHA. We use the recombinant Escherichia coli XL1-Blue to assess their use of processing waste water, and the ability for the synthesis of PHAs. Studies show the method can be used as a substrate in a variety of process wastewater. Besides, the generations of brewery processed wastewater from the cell growth and the PHB synthesis have significant results. Study also confirms that fermentation strategy without induction, Escherichia coli XL1-Blue synthetic PHB content can be accumulated to 8.87%. The study further shows: Escherichia coli XL1-Blue, if supplemented by a two-stage fermentation strategy, the PHB content can be further improved to 35%. It is evident that, using industrial process wastewater and with different fermentation strategies can effectively enhance the accumulation of PHAs content. The results show industrial process wastewater, used in synthetic and biological polymers PHA feasibility of reducing their production costs.
| Identifer | oai:union.ndltd.org:TW/099YZU05111115 |
| Date | January 2011 |
| Creators | Chih-Cheng Chiu, 邱志誠 |
| Contributors | Yu-Hong Wei, 魏毓宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 123 |
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