碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 100 / Antibiotics are one of the most commonly used chemicals in veterinary medicine for treating diseases. Sulfamethoxazole (SMX) and sulfadimethoxine (SDM) have been concern the aquatic environment due to the potential development of antibacterial resistance. In this study, we focused on the bacteria fauna involving the SMX and SDM biodegradation in two aquaculture pond sediments. Freshwater sediment samples were collected from an eel (Anguilla japonica) pond and seawater sediment samples were collected from a shrimp (Litopenaeus vannamei) pond. The sediment samples were spiked with SDM and SMX at concentration approximately of 50 mg L-1 and incubated in dark and anaerobic conditions at 25-28°C.
The half-lives (t1/2) were 10.3, 122.5, 76.4, 52.4 days for F-SMX (first added), S-SMX, F-SDM, and S-SDM, respectively. SMX had a fastest decline among the subsamples and was re-added into the freshwater sediment with a t1/2 of 13.3 days.
The results of PCR-DGGE and isolated pure culture of bacteria indicated that the Clostridium sp. was highly related to the decline of SMX and SDM in freshwater sediment and SMX in seawater sediment. Moreover, Bacillus sp. was related to decline of SDM in freshwater sediment. No identified bacteria predominant with the ability to decline SDM in seawater sediment.
| Identifer | oai:union.ndltd.org:TW/100061062 |
| Creators | Pisinee Aiumurai, 吳平平 |
| Contributors | Hong-Thih Lai, Ph. D., Chia-Wen Hsieh, Ph. D., 賴弘智, 謝佳雯 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 107 |
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