碩士 / 國立中正大學 / 化學工程研究所 / 100 / Due to rapidly global economic development, human heavy use of fossil fuels such as coal, oil and gas resulted in a substantial increase in carbon dioxide and greenhouse gases emissions, causing the destruction of the global carbon cycle and the global warming. One of possible solution to the problem of global warming is the use of microalgal photosynthesis for atmospheric carbon dioxide mitigation. Microalgaes can grow quickly and fix carbon dioxide to reduce carbon dioxide emission by manufacture factories; and because of its oil-rich they can be used for the production of renewable biofuel by extraction and transesterification. Thus the microalgal biofixation of carbon dioxide has considerable economic potential, and the ability to slow down the effects of global warming.
This study uses the photobioreactor culture to investigate the feeding effects on the light intensity and CO2 concentration of Nannochloropsus oculata. In the study, we found that microalgal biomass yield increased in 66.2% if the Walne’s medium were replased by modified Walne’s medium. Using the modified medium the biomass yield of N. oculata in fed-batch culture was 1.25 times higher than that in batch culture. Also, in the fed-batch culture the microalgal growth rate in modified Walne’s medium was 1.32 times higher than that in Walne’s medium. Light intensity was an important factor of microalgal growth in the high cell density culture. As the light intensity increased up to 2.5 times, microalgal growth increased from 4.1 to 29.4%, depending on the initial algal concentration. Under the conditions for high cell density culture, the highest microalgal biomass could be achieved in 2% CO2.
In this study, we also tried to construct a recombinant plasmid that could express the target gene encoding foreign protein in microalgae. The recombinant plasmid includes RBCs1 promoter, RBCs1 terminator and zeocin-resistance gene. In the study of N. oculata resistance to zeocin, we found that CC50 with N. oculata to zeocin concentration was 0.01-0.1 μg/ml. In the future, we can use 0.1 μg/ml zeocin for the screening of transgenic N. oculata based on these preliminary results.
| Identifer | oai:union.ndltd.org:TW/100CCU00063014 |
| Date | January 2012 |
| Creators | Shu-Hsien Chu, 朱書賢 |
| Contributors | Wen-Chien Lee, 李文乾 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 142 |
Page generated in 0.0091 seconds