碩士 / 嘉南藥理科技大學 / 生物科技系暨研究所 / 100 / The aim of this study was isolated from the freshwater and brackish water fish farms microalgae in southern Taiwan, and to explore the optimum growth conditions, the ability of lipid production and the phylogenetic relationship. Many applications are using microalgae such as cosmetics, health food, aquaculture feed, biomass energy or criminal identification. However, without higher magnification of the microscope and molecular identification, microalgae are not easy to identify. In this experiment, phylogenetic identification by ITS rDNA sequences was done for all fish farms microalgae. In addition, Desmodesmus and Scenedesmus were done by both 18S and ITS rDNA sequences. Samples waere collected from fish farm nearby Chia-Nan University, Su-Kunshen fish farm, Tu-Chia creek and Szu-Tsao wildlife reservation in southern Taiwan.
Fourteen microalgae were isolated, including 9 species of freshwater and 5 species of brackish water species. The results of 13 microalgal ITS rDNA were compared with the NCBI database. The phylogenetic trees were constructed using other known green algal 18S rDNA and ITS rDNA sequences. The tree topologies were obtained by Neighbor-Joining method, Maximum-likelihood method and Maximum-parsimony method. Comparing the ITS region rDNA sequence between 13 fish farms microalgae and 3 green aglae which kept in algal ecological laboratory were also found that the ITS region rDNA can be used as DNA barcodes in molecular identification. Moreover, the ITS2 rDNA sequence demonstrated better than ITS region rDNA(ITS1, 5.8S, ITS2)and 18S rDNA sequences in identify both genus Desmodesmus and genus Scenedesmus. The salinity tolerant studies indicated that freshwater microalgae can survive less than 1% salinity, and brackish water microalgae can survive less than 2% ~3%salinity. Nile red staining showed that only four green algae, including [Scenedesmus AENLCNU CENP-017], [Chlorella AENLCNU SQSP-023], [Chlorella AENLCNU CTUP-025] and [Chlorella AENLCNU DJCP-026] could increase the oil production potential by increasing the salinity concentration.
| Identifer | oai:union.ndltd.org:TW/100CNUP5111012 |
| Date | January 2012 |
| Creators | Wei-chien Hung, 洪維謙 |
| Contributors | Sheue-duan Lai, 賴雪端 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 128 |
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