碩士 / 中原大學 / 生物醫學工程研究所 / 100 / The wound is one of the main causes of bacterial infection and inflammation. Skin wound is common in various trauma and disease, such as abrasion, burn, diabetes, deceits and lasting-opened wound. A lasting-opened wound not only causes painfulness but also triggers invasion of microorganism. Dragon’s Blood is one kind of traditional Chinese medicine, which promotes wound healing and has been used to cover skin wound. The purpose of this research was to develop a wound dressing made with Dragon’s Blood, and investigate its function in wound healing.
The study included three parts. The first part was to investigate the basic properties of Dragon’s Blood. The concentration and functional group of Dragon’s Blood were measure by FT-IR and UV after the process of extraction. The second part was to make a composite dressing combining with Dragon’s Blood and alginate lyophilization. Liquid absorbance and solute loss of the composite dressing was then evaluated, according to China wound dressing testing method Y-K-K-23. Cytotoxicity was also investigated according to ISO 10993-5. Cytotoxicity of wound dressing made by Dragon’s Blood was investigated. The cytotoxicity test could analyze whether different concentration of Dragon’s Blood would stimulate or inhibit cell growth. Finally, anti-bacteria test was processed following AATCC 147. In the third part of wound healing experiment, 12 Rats with 6-week-old were used. A wound (2x2cm2) was cut on their back at three places. The wound healing effects of Dragon’s blood dressing, alginate dressing, and gauze were evaluated repeatedly at day six, day twelve and day eighteen.
In the experiment results, dracorhodin extraction from Dragon’s Blood showed similar FT-IR peak (1200nm) in Dragon’s Blood solution and Dragon’s Blood powder. In quantitative analysis, its absorption was proportional to concentration; however it could not be measured to light-sensitive material. Calcium chloride was added as a cross-linker in the manufacturing process. The cross-linked Dragon’s blood mixed dressing could absorb fluid with integrity. The absorption percentage was 700%. And it was proved that the dressing was cytotoxicity free, and provided good moisture environment for wound healing. The test result of AATCC 147 showed that it could inhibit Staphylococcus aureus growth. In animal experiment compared to control group, Dragon’s Blood dressing could promote wound healing on 12th day, which was 30% upper better than control.
Dragon’s Blood has been proved an effective material for wound healing. It has the properties of anti-inflammation and wound-recovery promotion. However, it is used as boil, powder and ointment to treat wound. These treatments cause second-time damage when replace the gauze. For our invention of Dragon’s Blood wound dressing, it not only kept the advantages of Dragon’s Blood, but also prevented contamination, promoted exudates absorption, protected wound and maintained moist environment for better wound healing.
| Identifer | oai:union.ndltd.org:TW/100CYCU5114002 |
| Date | January 2012 |
| Creators | Chia-Sheng Lin, 林嘉聖 |
| Contributors | Ching-Sung Weng, 翁清松 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 94 |
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