碩士 / 大葉大學 / 生物產業科技學系 / 100 / Trehalose synthase(Tsase) is easy and simple for the pathway in all trehalose synthsis way, used maltose to as substrate for change the bond of two glucose α, α 1-4 →α, α 1-1 bond, get the one trehalose molecular. Make use of PCR technology for numerous performance of the recombinant enzyme from E.coli. The permeabilization of microbial cells accompany with trehalose synthase activity were used to simplify trehalose production steps, increase its reusability and reduce the production cost for turtherindustrial applications.
The PTTS-D41P(Picrophilus torridus trehalose synthase -D41P) was a PTTS mutant in the study. Make use of point mutation technology according to the literature. Change the amino acid sequence of the aspartic acid to proline in the 41 site for improve of thermostability. Reduce production of cost and increase the production effcient, increase its for industrial production and business peddle.
| Identifer | oai:union.ndltd.org:TW/100DYU00111007 |
| Date | January 2012 |
| Creators | Shu-Ming Liou, 劉書銘 |
| Contributors | Shu-Wei Chang, 張淑微 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
Page generated in 0.5402 seconds