碩士 / 輔仁大學 / 生命科學系碩士班 / 99 / In response to climate change and fossil energy shortage, environmental sustainability and fuel substitute become more and more important to everyone on Earth. Therefore, the development of renewable energy is one of the important directions for further research. Among the different possible alternative energy source, oleaginous microorganisms play an important role in the biofuel industry. There are many species of yeasts and filamentous fungi have the capability to synthesize the oil drops in their cell. The purpose of this study was to isolated the wild yeasts strains from Taiwan coast and investigated their phylogenetic relationship. The molecular genetic approach by using RAPD biomarker and rRNA gene sequencing, and technique for detection lipid drops was using Nile Red stain. The 192 oleaginous microbes isolated from Taiwan coast were fallen into 11 groups by 5 random primer of RAPD. Analysis of 26S and ITS rRNA gene sequencing and the phylogenetic tree based on 3 different algorithms identified that there were 8 different genus and 13 different species in the 11 groups by RAPD. Although the ITS and 26S rRNA gene sequencing results of Rhodotorula mucilaginosa and Rhodosporidium paludigenum were too similar to be distinguished as separate species, there’s a definite classification result between them by the rapid-resolving resulting patterns from RAPD.
| Identifer | oai:union.ndltd.org:TW/100FJU00105002 |
| Date | January 2011 |
| Creators | Song, Zong-Han, 宋宗翰 |
| Contributors | Leu, Jyh-Yih, 呂誌翼 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
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