碩士 / 中華醫事科技大學 / 醫學檢驗生物技術系碩士班 / 100 / This study tries to make a comparison between ulva fasciata the largest class of green algae and chlorellaceae the smallest class of green algae by using different extraction methods, and also explores the effect of porphyra crispata kjellman that have not yet been developed in beauty and make-up field. To probe into the effect of algae on moisture retention, antioxidant activity and cell viability, chlorellaceae are extracted under 120℃ (chlo-lacⅠ) and 85℃ (chlo-lacⅡ) and settle in alcohol, while ulva fasciata are extracted under 120℃ and settle in alcohol (ulva fasciataⅠ) or freeze dried (ulva fasciataⅡ), por-cri oil are extracted from porphyra crispata kjellman.
Moisture retention tests cover hydration and transepidermal water loss (TEWL) values. Antioxidant activity tests involve diphenyl picryl hydrazinyl radical scavenging capacity, Fe2+-chelation ability, reducing power, cell viability and MTT assay.
In moisture retention tests, hydration results show that only chlorellaceae have hydrated moisture effect. Experimental results indicate the TEWL barrier function of the three kinds of marine algae. More significantly, ulva fasciataⅡ extracted by the freeze drying method are superior to ulva fasciata Ⅰ settled in alcohol in terms of moisture retention barrier effect.
In antioxidant activity tests under conditions of the 10mg/ml concentration, chlorellaceae Ⅰ and Ⅱ have almost the same DPPH scavenging rate of 67～71%; ulva fasciata Ⅰ is better than ulva fasciataⅡ.
In Fe2+ chelation ability tests under conditions of the 10mg/ml concentration, the chelation capabilities of chlorellaceae Ⅰ and Ⅱ are 65% and 63% respectively; ulva fasciata Ⅰ and Ⅱ’s chelation capabilities are 55% and 57% respectively, while porphyra crispata kjellman’ chelation capability reaches 66%. In reducing power tests, chlorellaceae Ⅱ is more powerful with 93% reducing power than chlorellaceaeⅠ with 55% reducing power. DPPH radical scavenging capacity and reducing power tests for ulva fasciata under the 10mg/ml concentration show that ulva fasciata Ⅱ have more scavenging capacity than ulva fasciata Ⅰ .
In cell viability tests of the algae extracts under the concentration of zero to 10mg/ml, results show that basically the viability of Ha Cat, B16 and Hela cells has few changes, indicating these extracts have hardly any killing effect on cells.
The above results provide a solid scientific basis for application of marine algae in beauty, anti-aging and clinical fields, and also confirm the feasibility of marine algae as cosmetics base materials.
| Identifer | oai:union.ndltd.org:TW/100HWAI7108003 |
| Date | January 2012 |
| Creators | YEH,YI CHUN, 葉怡君 |
| Contributors | Dong Zi Shao, Tai An Chiang, 邵東子, 姜泰安 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
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